Women's Health World Conference 2026

Abstract

Objective: To evaluate the short-term efficacy of a multi-strain probiotic supplement (Bifidobacterium longum, B. breve, B. lactis, B. animalis TA1, Lactobacillus paracasei MSMC 39-1, L. rhamnosus, L. reuteri TF314, and L. gasseri) in improving bowel movement frequency, stool consistency, and menopause-related quality of life in women with functional constipation.

Methods: A double-blind, randomized, placebo-controlled trial was conducted at the gynecology outpatient clinic of a tertiary care hospital in Bangkok, Thailand, between August 2024 and April 2025. For four weeks, 46 women with functional constipation, as defined by Rome IV, were randomized to receive a probiotic or placebo capsule every day. Simple randomization was carried out by a third party, and the allocation was hidden from the participants and researchers. Pretest–posttest questionnaires were used to measure menopause-specific quality of life (MENQOL) and symptoms associated with constipation. Data were analyzed using the intention-to-treat approach.

Results: In the probiotics group, the median weekly defecation frequency increased significantly (3.0 to 5.0, p=0.003). Hard stools slightly declined from 32% to 9.1% (p=0.200), although not statistically significant (p = 0.200).  There were minimal changes in the placebo group. The total MENQOL score, and physical domain improved from 55.1±31.2 to 40.7±22.4 (p=0.012) and from 34.2±21.5 to 24.4±14.1 (p=0.014), respectively, while vasomotor, psychosocial, and sexual domains showed no significant changes.  No significant changes occurred in the placebo group. Subgroup analysis of participants over 60 years old showed similar improvements in total score (p=0.041) and physical domain (p=0.029). One participant (4.3%) in the probiotics group discontinued due to bloating.

Conclusions: In menopausal women experiencing constipation, a 4-week multi-strain probiotic treatment demonstrated improvements in the frequency of bowel movements, physical domain and total score of menopause-related quality of life. Probiotics may serve as an adjunctive, non-pharmacological approach to support physical well-being in menopausal women.